- Thermostable reverse transcriptase 45°C to 55°C
- Contains advanced RNase inhibitor
- Non-PCR inhibiting intercalating dye
- Rapid extension rate for early Ct values
- Market leading sensitivity – increased limit of detection
- Compatible on all real-time PCR platforms – standard and fast cycling conditions
qPCRBIO 1-Step SyGreen Detect and Go mixes use the latest developments in reverse transcriptase technology and buffer chemistry for efficient cDNA synthesis and qPCR in a single tube. The qPCR is performed by PCRBIO Taq HS DNA Polymerase, resulting in robust RT-PCR performance.
qPCRBIO 1-Step SyGreen Detect mix can be used to quantify any RNA template including mRNA, total RNA and viral sequences. Extremely low copy number targets can be detected specifically with high efficiency. Antibody-mediated hot start technology prevents formation of primer dimers and non-specific products leading to improved reaction sensitivity and specificity. Combining the latest advancements in polymerase technology and advanced buffer chemistry we offer market leading performance with minimal or no optimisation. qPCRBIO SyGreen 1-Step Detect recommended for template amounts of 1pg – 10ng total RNA or >0.01pg mRNA per reaction.
qPCRBIO 1-Step SyGreen Go mix can be used to quantify any RNA template including mRNA, total RNA and viral sequences. Extremely low copy number targets can be detected specifically with high efficiency. The mix has been optimised for speed (early Ct) for rapid detection. qPCRBIO SyGreen 1-Step Go recommended for template amounts of 10pg – 100ng total RNA or >0.01pg mRNA per reaction.
Many modern real-time PCR instruments have a fast cycling mode. The same developments that improve the sensitivity and consistency of qPCRBIO SyGreen mix in standard cycling conditions also allow for industry leading performance in fast and ultra fast cycling conditions.